NSI-189

NSI-189 is an oral investigational compound that promotes hippocampal neurogenesis, utilized off-label primarily for mood improvement and cognitive enhancement. Users typically run a daily dose of 40 mg, though reported intake ranges widely from 200 µg to 50 mg without a standardized cycling protocol. While users occasionally report changes in sleep patterns and dream intensity, the primary tradeoff is that the evidence base for these human applications remains thin, as the compound lacks FDA approval and most exploratory research lacks published results. Injection-site reactions, histamine responses, and gastrointestinal issues have not been a consistent theme in community reports.

Half-life~18 h

Data last updated

ClinicalResearchDepthClinicalResearchEfficacyClinicalResearchSideEffectsno dataAnecdotalEfficacyAnecdotalSideEffectsPrice2.71.73.53.12.0

Measured on 5 of 6 axes

Research Evidence

Evidence shape

NSI-189 currently sits at a low evidence tier, as there are no published human studies to support its use. While four trials have been registered—including two completed studies focused on depression and major depressive disorder—the research remains confined to early-stage, exploratory phases and preclinical animal models. The absence of published results from these completed trials limits the ability to verify efficacy or safety, leaving the peptide’s clinical profile largely uncharacterized beyond its initial mechanistic research.

Depthhow much
4 registered trials
2 completed
Breadthhow many areas
4 indications mapped
3 thin / exploratory · 1 animal-only · 1 single-trial long-tail
Qualityhow rigorous
Highest tier: Phase 1
1 linked publication on registered trials
Breadth and depth11 rows captured
Human
direct clinical signal
5
Animal
translational support
6
In vitro
mechanistic support
0
HighMediumLow

Anecdotal efficacy

1
USA
40 mg
$37.99
$0.95/mg
2
GB
20 mg
$80.52
$4.03/mg

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NSI-189 dosing & protocol

How NSI-189 is dosed across research, clinician, and community sources — each evidence tier kept separate so the dose range, frequency, timing, and cycling stay visible without flattening different levels of evidence.

Research trials
Published clinical-trial protocols · n = 2
Typical dose
0
10 mg
20 mg
30 mg
40 mg
50 mg
no dose captured for this tier yet
Frequency
daily
Route
oral

The two completed trials of NSI‑189 did not provide a reliably extracted dose; participants received the peptide orally each day, but the exact milligram amount per administration is not reported.

2 sources
Clinician practice
Doctor & published-protocol guidance
Typical dose
0
10 mg
20 mg
30 mg
40 mg
50 mg
no dose captured for this tier yet

No structured protocol details captured for this tier yet.

Anecdotal
Community-reported real-world use · n = 24
Typical dose
40 mg200 µg – 50 mg
typical
0
10 mg
20 mg
30 mg
40 mg
50 mg
distribution of 29 reported doses · darker = more
By stated route· tap a row to filter the chart
Frequency
unspecified
Route
oral

Community reports center around 40 mg taken orally, with most sources falling in the 20–80 mg range; a smaller subset reports doses of 200–600 mcg. Dosing frequency was not consistently specified across these reports.

24 sources